recombinant mouse stem cell factor (mscf, cat Search Results


viobright fitc  (Miltenyi Biotec)
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Miltenyi Biotec viobright fitc

Viobright Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mscv hygro pgk hygro vector  (TaKaRa)
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TaKaRa mscv hygro pgk hygro vector
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recombinant dna pmscv cre puro ires gfp addgene plasmid  (Addgene inc)
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Addgene inc recombinant dna pmscv cre puro ires gfp addgene plasmid
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rt pcr primers n a n a recombinant dna mscv ires thy1 1 addgene plasmid id  (Addgene inc)
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Rt Pcr Primers N A N A Recombinant Dna Mscv Ires Thy1 1 Addgene Plasmid Id, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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600af647 bacterial  (Addgene inc)
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Addgene inc 600af647 bacterial
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600af647 Bacterial, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse stem cell factor mscf  (R&D Systems)
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R&D Systems mouse stem cell factor mscf
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Mouse Stem Cell Factor Mscf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant murine scf  (R&D Systems)
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recombinant murine scf rmscf  (R&D Systems)
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mouse stem cell signaling rt2 profiler pcr arrays  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc mouse stem cell signaling rt2 profiler pcr arrays
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Mouse Stem Cell Signaling Rt2 Profiler Pcr Arrays, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantitative colorimetric peptide assay thermo fisher scientific  (Addgene inc)
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sci rep 201649 addgene plasmid  (Addgene inc)
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52961 mscv gfp dr patrick seale na mscv prdm16 dr patrick seale na software  (Addgene inc)
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Addgene inc 52961 mscv gfp dr patrick seale na mscv prdm16 dr patrick seale na software
(A) Western blot analysis of <t>PRDM16,</t> PPARγ, and α-Actin protein levels in whole iWAT (left) or in isolated adipocytes and stromal-vascular cells (SVC) from iWAT of young and aged mice acclimated to thermoneutrality.
52961 Mscv Gfp Dr Patrick Seale Na Mscv Prdm16 Dr Patrick Seale Na Software, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Cell Stem Cell

Article Title: Human naive epiblast cells possess unrestricted lineage potential

doi: 10.1016/j.stem.2021.02.025

Figure Lengend Snippet:

Article Snippet: SUSD2 Antibody, anti-human, VioBright FITC , Miltenyi Biotech , Cat#130-106-401; RRID: AB_2653618.

Techniques: Recombinant, Cell Attachment Assay, Transfection, Plasmid Preparation, Software

KEY RESOURCES TABLE

Journal: Cancer cell

Article Title: Vitamin B6 addiction in acute myeloid leukemia

doi: 10.1016/j.ccell.2019.12.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The full length of mouse or human PDXK cDNA was cloned into MSCV Hygro-PGK-Hygro vector (Clontech 634401).

Techniques: Plasmid Preparation, Recombinant, Mutagenesis, RNA Extraction, Gel Extraction, Clone Assay, Purification, Flow Cytometry, Sequencing, CRISPR, Functional Assay, shRNA, Software

(A) Western blot analysis of PRDM16, PPARγ, and α-Actin protein levels in whole iWAT (left) or in isolated adipocytes and stromal-vascular cells (SVC) from iWAT of young and aged mice acclimated to thermoneutrality.

Journal: Cell metabolism

Article Title: A PRDM16-driven metabolic signal from adipocytes regulates precursor cell fate

doi: 10.1016/j.cmet.2019.05.005

Figure Lengend Snippet: (A) Western blot analysis of PRDM16, PPARγ, and α-Actin protein levels in whole iWAT (left) or in isolated adipocytes and stromal-vascular cells (SVC) from iWAT of young and aged mice acclimated to thermoneutrality.

Article Snippet: Aged mice (+/− CL316,243) -Control (Puro) vs. PRDM16-expressing adipose cells GEO repository {"type":"entrez-geo","attrs":{"text":"GSE129083","term_id":"129083"}} GSE129083 {"type":"entrez-geo","attrs":{"text":"GSE129084","term_id":"129084"}} GSE129084 Experimental Models: Organisms/Strains R26 CreER The Jackson Laboratory 004847 Prdm16 flox The Jackson Laboratory 024992 Bdh1 flox Dr. Daniel Kelly NA Adipoq Cre The Jackson Laboratory 010803 Rosa26 tdTomato The Jackson Laboratory 007914 Pdgfrα creERT2 Dr. Brigid Hogan NA Adipoq rtTA Dr. Philipp Scherer NA Tre Cre The Jackson Laboratory 006234 Rosa26 mTmG The Jackson Laboratory 007676 Prdm16-deficient Dr. Patrick Seale NA Fabp4-Prdm16 Dr. Bruce Spiegelman NA Oligonucleotides See Table S1 for qPCR Primers Integrated DNA Technologies (IDT) NA Recombinant DNA PLKO.1-shRNA-Scramble Addgene, Sabatini, PMID15718470 1864 PLKO.1-shRNA-Bdh1 The RNAi Consortium (TRC)- Broad Institute via UPenn High-Throughput Screening Core TRCN0000041898 pMD2.G Addgene (Gift from Didier Trono) 12259 psPAX2 Addgene (Gift from Didier Trono) 12260 LentiCRISPR v2 Addgene (Gift from Feng Zhang) 52961 MSCV-GFP Dr. Patrick Seale NA MSCV-Prdm16 Dr. Patrick Seale NA Software and Algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/prism/ Image J NIH https://imagej.nih.gov/ij/ R Open source https://www.r-project.org/ GSEA Broad Institute http://software.broadinstitute.org/gsea/index.jsp Reactome (Pathway Browser) PMID: 29145629 https://reactome.org/PathwayBrowser/ Open in a separate window KEY RESOURCES TABLE Beiging stimuli decrease the fibrotic phenotype of adipose precursor cells.

Techniques: Western Blot, Isolation

(A-D) iWAT precursor cells were transduced with control (CTL) or PRDM16-expressing retrovirus and treated with either vehicle control (CTL) or DMOG. After 2 days, cells were immunostained for ACTA2 or induced to differentiate into adipocytes for 5 days.

Journal: Cell metabolism

Article Title: A PRDM16-driven metabolic signal from adipocytes regulates precursor cell fate

doi: 10.1016/j.cmet.2019.05.005

Figure Lengend Snippet: (A-D) iWAT precursor cells were transduced with control (CTL) or PRDM16-expressing retrovirus and treated with either vehicle control (CTL) or DMOG. After 2 days, cells were immunostained for ACTA2 or induced to differentiate into adipocytes for 5 days.

Article Snippet: Aged mice (+/− CL316,243) -Control (Puro) vs. PRDM16-expressing adipose cells GEO repository {"type":"entrez-geo","attrs":{"text":"GSE129083","term_id":"129083"}} GSE129083 {"type":"entrez-geo","attrs":{"text":"GSE129084","term_id":"129084"}} GSE129084 Experimental Models: Organisms/Strains R26 CreER The Jackson Laboratory 004847 Prdm16 flox The Jackson Laboratory 024992 Bdh1 flox Dr. Daniel Kelly NA Adipoq Cre The Jackson Laboratory 010803 Rosa26 tdTomato The Jackson Laboratory 007914 Pdgfrα creERT2 Dr. Brigid Hogan NA Adipoq rtTA Dr. Philipp Scherer NA Tre Cre The Jackson Laboratory 006234 Rosa26 mTmG The Jackson Laboratory 007676 Prdm16-deficient Dr. Patrick Seale NA Fabp4-Prdm16 Dr. Bruce Spiegelman NA Oligonucleotides See Table S1 for qPCR Primers Integrated DNA Technologies (IDT) NA Recombinant DNA PLKO.1-shRNA-Scramble Addgene, Sabatini, PMID15718470 1864 PLKO.1-shRNA-Bdh1 The RNAi Consortium (TRC)- Broad Institute via UPenn High-Throughput Screening Core TRCN0000041898 pMD2.G Addgene (Gift from Didier Trono) 12259 psPAX2 Addgene (Gift from Didier Trono) 12260 LentiCRISPR v2 Addgene (Gift from Feng Zhang) 52961 MSCV-GFP Dr. Patrick Seale NA MSCV-Prdm16 Dr. Patrick Seale NA Software and Algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/prism/ Image J NIH https://imagej.nih.gov/ij/ R Open source https://www.r-project.org/ GSEA Broad Institute http://software.broadinstitute.org/gsea/index.jsp Reactome (Pathway Browser) PMID: 29145629 https://reactome.org/PathwayBrowser/ Open in a separate window KEY RESOURCES TABLE Beiging stimuli decrease the fibrotic phenotype of adipose precursor cells.

Techniques: Transduction, Expressing

(A) Gene Set Enrichment Analysis (GSEA) of genes upregulated in PRDM16-expressing vs. control cells. Enriched pathways were defined by a normalized enrichment score > 1.3 and FDR < 0.05.

Journal: Cell metabolism

Article Title: A PRDM16-driven metabolic signal from adipocytes regulates precursor cell fate

doi: 10.1016/j.cmet.2019.05.005

Figure Lengend Snippet: (A) Gene Set Enrichment Analysis (GSEA) of genes upregulated in PRDM16-expressing vs. control cells. Enriched pathways were defined by a normalized enrichment score > 1.3 and FDR < 0.05.

Article Snippet: Aged mice (+/− CL316,243) -Control (Puro) vs. PRDM16-expressing adipose cells GEO repository {"type":"entrez-geo","attrs":{"text":"GSE129083","term_id":"129083"}} GSE129083 {"type":"entrez-geo","attrs":{"text":"GSE129084","term_id":"129084"}} GSE129084 Experimental Models: Organisms/Strains R26 CreER The Jackson Laboratory 004847 Prdm16 flox The Jackson Laboratory 024992 Bdh1 flox Dr. Daniel Kelly NA Adipoq Cre The Jackson Laboratory 010803 Rosa26 tdTomato The Jackson Laboratory 007914 Pdgfrα creERT2 Dr. Brigid Hogan NA Adipoq rtTA Dr. Philipp Scherer NA Tre Cre The Jackson Laboratory 006234 Rosa26 mTmG The Jackson Laboratory 007676 Prdm16-deficient Dr. Patrick Seale NA Fabp4-Prdm16 Dr. Bruce Spiegelman NA Oligonucleotides See Table S1 for qPCR Primers Integrated DNA Technologies (IDT) NA Recombinant DNA PLKO.1-shRNA-Scramble Addgene, Sabatini, PMID15718470 1864 PLKO.1-shRNA-Bdh1 The RNAi Consortium (TRC)- Broad Institute via UPenn High-Throughput Screening Core TRCN0000041898 pMD2.G Addgene (Gift from Didier Trono) 12259 psPAX2 Addgene (Gift from Didier Trono) 12260 LentiCRISPR v2 Addgene (Gift from Feng Zhang) 52961 MSCV-GFP Dr. Patrick Seale NA MSCV-Prdm16 Dr. Patrick Seale NA Software and Algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/prism/ Image J NIH https://imagej.nih.gov/ij/ R Open source https://www.r-project.org/ GSEA Broad Institute http://software.broadinstitute.org/gsea/index.jsp Reactome (Pathway Browser) PMID: 29145629 https://reactome.org/PathwayBrowser/ Open in a separate window KEY RESOURCES TABLE Beiging stimuli decrease the fibrotic phenotype of adipose precursor cells.

Techniques: Expressing

KEY RESOURCES TABLE

Journal: Cell metabolism

Article Title: A PRDM16-driven metabolic signal from adipocytes regulates precursor cell fate

doi: 10.1016/j.cmet.2019.05.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Aged mice (+/− CL316,243) -Control (Puro) vs. PRDM16-expressing adipose cells GEO repository {"type":"entrez-geo","attrs":{"text":"GSE129083","term_id":"129083"}} GSE129083 {"type":"entrez-geo","attrs":{"text":"GSE129084","term_id":"129084"}} GSE129084 Experimental Models: Organisms/Strains R26 CreER The Jackson Laboratory 004847 Prdm16 flox The Jackson Laboratory 024992 Bdh1 flox Dr. Daniel Kelly NA Adipoq Cre The Jackson Laboratory 010803 Rosa26 tdTomato The Jackson Laboratory 007914 Pdgfrα creERT2 Dr. Brigid Hogan NA Adipoq rtTA Dr. Philipp Scherer NA Tre Cre The Jackson Laboratory 006234 Rosa26 mTmG The Jackson Laboratory 007676 Prdm16-deficient Dr. Patrick Seale NA Fabp4-Prdm16 Dr. Bruce Spiegelman NA Oligonucleotides See Table S1 for qPCR Primers Integrated DNA Technologies (IDT) NA Recombinant DNA PLKO.1-shRNA-Scramble Addgene, Sabatini, PMID15718470 1864 PLKO.1-shRNA-Bdh1 The RNAi Consortium (TRC)- Broad Institute via UPenn High-Throughput Screening Core TRCN0000041898 pMD2.G Addgene (Gift from Didier Trono) 12259 psPAX2 Addgene (Gift from Didier Trono) 12260 LentiCRISPR v2 Addgene (Gift from Feng Zhang) 52961 MSCV-GFP Dr. Patrick Seale NA MSCV-Prdm16 Dr. Patrick Seale NA Software and Algorithms GraphPad Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/prism/ Image J NIH https://imagej.nih.gov/ij/ R Open source https://www.r-project.org/ GSEA Broad Institute http://software.broadinstitute.org/gsea/index.jsp Reactome (Pathway Browser) PMID: 29145629 https://reactome.org/PathwayBrowser/ Open in a separate window KEY RESOURCES TABLE Beiging stimuli decrease the fibrotic phenotype of adipose precursor cells.

Techniques: Generated, Recombinant, Sample Prep, SYBR Green Assay, RNA Sequencing Assay, Mouse Assay, Software